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KMID : 0043320110340050741
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Archives of Pharmacal Research
2011 Volume.34 No. 5 p.741 ~ p.746
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High-performance liquid chromatographic determination and pharmacokinetic study of apigenin-7-O-β-D-glucoside in rat plasma after intravenous administration
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Chen Zaixing
Ying Xixiang
Meng Shu
Zhu Xu
Jiang Hong
Cao Qishen
Li Xuying
Meng Fanhao
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Abstract
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The present study was designed to validate an analytical method based on HPLC for the quantitative measurement of the Apigenin-7-O-β-D-glucoside (AGL) in rat plasma after intravenous administration. HPLC analysis was done using a C18 column, UV detection at 335 nm, and a mobile phase of methanol:0.2% phosphoric acid (1:1, v/v). Good linearity was observed over the range of 0.06?7.20 μg/mL with a lower limit of quantication of 0.06 μg/mL. The intraand inter-day precision values were below 9.97%. No interference peaks or matrix effects were observed. The method was fully validated and successfully applied to a pharmacokinetic study of AGL.
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KEYWORD
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Apigenin-7-O-β-D-glucoside, Pharmacokinetics, High performance liquid chromatography
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